| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2008-01-08 |
| タイトル |
|
|
タイトル |
In Vitro Phosphorylation of Proteins in IAA-Treated Primary Roots and Coleoptiles in Zea mays |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
Kato, Ryoichi
Takatsuna, Sachiko
Wada, Tsuyoshi
Narihara, Yumi
Suzuki, Takashi
|
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
9141 |
|
|
姓名 |
加藤, 良一 |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Five-mm sections of elongation zones which were cut from primary roots or coleoptiles of Zea mays were incubated for designated periods with various concentrations of IAA. In vitro protein phosphorylation in the soluble fractionsprepared from these sections was analyzed by SDS-PAGE. The phosphorylation of proteins in sections of primary roots incubated for 20 or 40 min in the presenceof 10^-7 M IAA was greater than that in the sections incubated in the absence ofIAA. The phosphorylation of proteins in sections of primary roots incubated for 20 min or 2h in the presence of 10^-8,10^-7 or 10^-6 M IAA was higher than that in the sections incubated in the absence of IAA. An incuba-tion for 20 min or 2h with 10^-4 M IAA inhibited the phosphorylation of proteins in sections of primary roots. The growth of the sections of primary roots incubated for 2h in the presence of 10^-7 M IAA or higher concentrations was lower than that of the sections incubated in the absence of IAA. These results suggest that the phosphorylation of proteins which was increased by IAA treatment is independent of an inhibition of the growth induced by IAA in maize primary roots. The phosphorylation of proteins in sections of coleoptiles incubated for 10, 20 or 40 min in the presence of 10^-7 or 10^-5 M IAA was equal to that in the sectionsincubated in the absence of IAA. These results show that IAA regulates growth ofmaize coleoptiles in no relation with phosphorylation of the proteins and that IAA regulates growth of maize mesocotyls via phosphorylation of the proteins, comparing with earlier results [Kato et al. (1996) Plant Cell Phsiol. 37:667]. Coleoptile―IAA treatment―Primary root―Protein phosphorylation―Zea mays. |
| 書誌情報 |
山形大学紀要. 自然科学 = Bulletin of Yamagata University. Natural Science
巻 15,
号 3,
p. 89-104,
発行日 2003-02-17
|
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN0024300X |
| 出版者 |
|
|
出版者 |
山形大学 |
| 出版者（ヨミ） |
|
|
値 |
ヤマガタ ダイガク |
| 別言語の出版者 |
|
|
値 |
Yamagata University |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文(Article) |
| 資源タイプ・ローカル |
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|
値 |
紀要論文 |
| 資源タイプ・NII |
|
|
値 |
Departmental Bulletin Paper |
| 資源タイプ・DCMI |
|
|
値 |
text |
| 資源タイプ・ローカル表示コード |
|
|
値 |
2 |
kiyoun-15-3-089to104.pdf (2.1 MB)
