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Translocation of cationic polystyrene microspheres associated with actin filaments on a surface coated with myosin motors
https://yamagata.repo.nii.ac.jp/records/3958
https://yamagata.repo.nii.ac.jp/records/39583d553fd8-4477-46e8-b6e6-f517bce4595c
名前 / ファイル | ライセンス | アクション |
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kiyoue-36-001to011.pdf (2.8 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-01-18 | |||||
タイトル | ||||||
タイトル | Translocation of cationic polystyrene microspheres associated with actin filaments on a surface coated with myosin motors | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
OYAMA, Takuya
× OYAMA, Takuya× HATORI, Kuniyuki |
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著者別名 | ||||||
姓名 | 小山, 拓也 | |||||
著者別名 | ||||||
姓名 | 羽鳥, 晋由 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Muscular actin filaments can glide on a myosin-fixed glass surface by mechanochemical energy transduction. Here, we examined whether artificial objects bound to actin filaments can be translocated actively by myosin motors. Actin molecules may have a monomeric (G-actin) or fibrous form (F-actin), depending on the ionic conditions. Each form of actin molecules was mixed with a suspension of polystyrene microspheres carrying amine groups. After myosin motors were fixed on the collodion-coated glass surface, the mixture of actin molecules and microspheres was applied in the presence of adenosine triphosphate. The motion of the microspheres was monitored under a phase-contrast optical microscope. The microspheres mixed with G-actin exhibited thermal fluctuations due to insufficient binding of G-actin. In contrast, F-actin could bind to the microspheres and they were translocated in linear manner at approximately 1.5 μm/s on the myosin-coated surface. These results demonstrate that the actin filaments bound to polystyrene microspheres are capable of translocation between the actin and myosin. | |||||
書誌情報 |
山形大学紀要. 工学 en : Bulletin of Yamagata University. Engineering 巻 36, p. 1-11, 発行日 2016-01 |
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書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00242991 | |||||
出版者 | ||||||
出版者 | 山形大学 | |||||
出版者(ヨミ) | ||||||
ヤマガタ ダイガク | ||||||
別言語の出版者 | ||||||
Yamagata University | ||||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | 論文(Article) | |||||
資源タイプ・ローカル | ||||||
紀要論文 | ||||||
資源タイプ・NII | ||||||
Departmental Bulletin Paper | ||||||
資源タイプ・DCMI | ||||||
text | ||||||
資源タイプ・ローカル表示コード | ||||||
2 |