{"created":"2023-05-25T05:22:51.634941+00:00","id":182,"links":{},"metadata":{"_buckets":{"deposit":"65139b40-6324-40e8-8bcc-d7980b1d7bfb"},"_deposit":{"created_by":3,"id":"182","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"182"},"status":"published"},"_oai":{"id":"oai:yamagata.repo.nii.ac.jp:00000182","sets":["29:31:130:132"]},"author_link":["610","612","611"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-02-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"29","bibliographicPageStart":"23","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"山形大学紀要. 医学 : 山形医学 = Bulletin of the Yamagata University. Medical science : Yamagata medical journal"}]}]},"item_3_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Abstruct MGSAD023 (Mouse Gene Similar to AD023; Genbank accession AK150749) and its human compartment (AD023; Genbank accession AF225422) are new two genes with unknown functions. They were derived from high-throughput cDNA sequence analysis of the mouse genome project and Human Genome Project (HGP)，respec- tively. MGSAD023 shows 88.6% identity to AD023 (NCBI Blast)，and we decided to use MGSAD023 as a temple to explore the functions of these two compartment genes. With the NCBI Conserved Domain Database (CDD) and the gap program in GCG se- quence analysis software，we found that the amino acids of MGSAD023 showed 58.3% similarity and 4l.2% identity to eIF4G and also showed a good match to the middle domain of eIF4G. We determined the transcription pattern of MGSAD023. RT-PCR results showed that MGSAD023 was expressed in all of the tissues checked，including cerebellum， cerebra，liver，kidney，tongue，testis，heart and spleen，without any significant differ- ences.The results of In Situ Hybridization (ISH) showed that there was a very strong signal of mRNA of MGSAD023 on the seminiferous tubular basement membrane， where spermatogonia A and B are located. In silico exploration revealed homologous genes in various species，i ncluding chimpanzee，rat，mice，dog，zebra fish，fruit fly， xenopus，and cattle. These results hint that MGSAD023 is a conserved gene. It may act as a translation factor during cellular mitosis，especially in spermatogonia.","subitem_description_type":"Other"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"山形大学"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00067279","subitem_source_identifier_type":"NCID"}]},"item_3_text_33":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ヤマガタ ダイガク"}]},"item_3_text_34":{"attribute_name":"別言語の出版者","attribute_value_mlt":[{"subitem_text_value":"Yamagata University"}]},"item_3_text_43":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_3_text_44":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_3_text_45":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_3_text_46":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Pan,  Lei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto,  Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura,  Wataru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-08-21"}],"displaytype":"detail","filename":"kiyoum-27-1-023to029.pdf","filesize":[{"value":"280.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kiyoum-27-1-023to029.pdf","url":"https://yamagata.repo.nii.ac.jp/record/182/files/kiyoum-27-1-023to029.pdf"},"version_id":"b9d22e16-b004-4099-820b-aae299d5f5a2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Primary research on MGSAD023, a new homokogue of eIF4G","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Primary research on MGSAD023, a new homokogue of eIF4G"}]},"item_type_id":"3","owner":"3","path":["132"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-25"},"publish_date":"2010-06-25","publish_status":"0","recid":"182","relation_version_is_last":true,"title":["Primary research on MGSAD023, a new homokogue of eIF4G"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-25T06:30:19.769660+00:00"}