{"created":"2023-05-25T05:24:31.481373+00:00","id":2360,"links":{},"metadata":{"_buckets":{"deposit":"238f8693-a5fc-4433-9d1f-5559a5a7fc8f"},"_deposit":{"created_by":3,"id":"2360","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2360"},"status":"published"},"_oai":{"id":"oai:yamagata.repo.nii.ac.jp:00002360","sets":["64:67:213:242"]},"author_link":["7867","7872","7869","7870","7866","7873","7871","7868"],"item_3_alternative_title_18":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"An Alternative to 'Peeling Method' for the Isolation of Leaf Protoplasts in Lycopersicon and Solanum"}]},"item_3_alternative_title_19":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ハ プロトプラスト ノ タンリ ニ オケル ヒョウヒ ハクヒホウ ニ カワル ホウホウ ニツイテ"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-01-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"414","bibliographicPageStart":"409","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"山形大学紀要. 農学 = Bulletin of the Yamagata University. Agricultural science"}]}]},"item_3_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"【緒論】葉を用いてプロトプラストを単離する場合，酵素処理において業の内部へ酵素液を浸透させる必要がある.そのため，葉の裏側を剥皮し，剥皮した側を下にして酵素液に浮かべる方法(表皮剥皮法)と葉を細断し酵素液に浸す方法(葉細断法)が用いられる.後者の場合，酵素液に葉を浸したのち真空ポンプを用いて葉の内部の気体を抜く必要がある.気体抜きをしないとプロトプラスト収量が著しく減少する.トマト属の葉プロトプラストを単離する場合，子葉では表皮剥皮方法を容易に適用することが出来るが,本葉では裏側表皮の剥皮はその操作が技術的に非常に困難である.したがって，一般的に葉細断法が用いられている.葉細断法の短所は気体抜きのための無菌条件下での設備と時間が入用であり，さらに，脱気の程度に関する真空ポンプ操作上の経験を必要とする.本報告では表皮剥皮法や葉細断法に代わる方法について述べる.","subitem_description_type":"Other"}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"7870","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Imanishi,  Shigeru"}]},{"nameIdentifiers":[{"nameIdentifier":"7871","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Egashira,  Hiroaki"}]},{"nameIdentifiers":[{"nameIdentifier":"7872","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hirono,  Naoyoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"7873","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Satoh,  Kenji"}]}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"山形大学"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00243054","subitem_source_identifier_type":"NCID"}]},"item_3_text_33":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ヤマガタ ダイガク"}]},"item_3_text_34":{"attribute_name":"別言語の出版者","attribute_value_mlt":[{"subitem_text_value":"Yamagata University"}]},"item_3_text_43":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_3_text_44":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_3_text_45":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_3_text_46":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今西,  茂"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"江頭,  宏昌"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"広野,  直芳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐藤,  健二"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-08-24"}],"displaytype":"detail","filename":"kiyoua-11-2-409to414.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kiyoua-11-2-409to414.pdf","url":"https://yamagata.repo.nii.ac.jp/record/2360/files/kiyoua-11-2-409to414.pdf"},"version_id":"6b63aa2a-efae-4847-9655-72085c0a456c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"葉プロトプラストの単離における表皮剥皮法に代わる方法について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"葉プロトプラストの単離における表皮剥皮法に代わる方法について"}]},"item_type_id":"3","owner":"3","path":["242"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-23"},"publish_date":"2012-10-23","publish_status":"0","recid":"2360","relation_version_is_last":true,"title":["葉プロトプラストの単離における表皮剥皮法に代わる方法について"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-25T06:13:14.285212+00:00"}