{"created":"2023-05-25T05:25:43.969872+00:00","id":4211,"links":{},"metadata":{"_buckets":{"deposit":"ab6e5f2b-6d61-4389-9625-0190ac7cdc2d"},"_deposit":{"created_by":17,"id":"4211","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"4211"},"status":"published"},"_oai":{"id":"oai:yamagata.repo.nii.ac.jp:00004211","sets":["29:31:100:101"]},"author_link":["13164","13162","13156","13163","13159","13157","13161","13158","13160"],"item_3_alternative_title_18":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"フローサイトメトリーを用いたシグナル伝達因子の解析は収束性シグナルと分散性シグナルの検出に有用である"}]},"item_3_alternative_title_19":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of signal transducers using flow cytometry is useful for detection of contractive and fluctuating signals"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-08-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"32","bibliographicPageStart":"21","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"山形大学紀要. 医学 : 山形医学"},{"bibliographic_title":"Bulletin of the Yamagata University. Medical science : Yamagata medical journal","bibliographic_titleLang":"en"}]}]},"item_3_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"論文(Article)","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"　Investigation of signal transduction mechanisms is important for development of therapy and understanding complex life systems. In this study, to establish a novel recognition method of signal transduction pathways, we investigated signal transducers using flow cytometry.\n　The flow cytometric measurement shows a mean phosphorylation level (mean of fluorescence intensity, MFI) and a deviation of the phosphorylation level (coefficient variation, CV) in a cluster of cells. As a model of signal pathways, Jurkat cells (T cell leukemia cell line) were stimulated with interleukin-21 or interferon-α, and signal transducers and activators of transcription (STATs) and extracellular signal-regulated kinase (ERK) 1/2 were measured using flow cytometry. Furthermore, peripheral blood was stimulated, and then various signal transducers of the lymphocytes and neutrophils were analyzed with MFI and CV.\n　After the stimulation, the increase of STATs MFI induced a temporal change of CV. On the other hand, the decrease of ERK1/2 phosphorylation accompanied the sustained increase of CV. Finally, we classified the signaling characters into five types using a combination of MFI and CV. These findings contribute to an explanation of the known relationship between signal transducers and stimulants on each cell subset. Therefore, this method may be useful to discover a causal relationship between stimulants and signal transducers in complex systems.","subitem_description_type":"Other"}]},"item_3_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"13159","nameIdentifierScheme":"WEKO"}],"names":[{"name":"タケダ, ユウジ"}]},{"nameIdentifiers":[{"nameIdentifier":"13160","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ナラ, ヒデトシ"}]},{"nameIdentifiers":[{"nameIdentifier":"13161","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アサオ, ヒロノブ"}]}]},"item_3_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"13162","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takeda, Yuji"}]},{"nameIdentifiers":[{"nameIdentifier":"13163","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nara, Hidetoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"13164","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Asao, Hironobu"}]}]},"item_3_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15022/00004195","subitem_identifier_reg_type":"JaLC"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"山形大学"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0288-030X","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00067279","subitem_source_identifier_type":"NCID"}]},"item_3_text_33":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ヤマガタ ダイガク"}]},"item_3_text_34":{"attribute_name":"別言語の出版者","attribute_value_mlt":[{"subitem_text_value":"Yamagata University"}]},"item_3_text_43":{"attribute_name":"資源タイプ・ローカル","attribute_value_mlt":[{"subitem_text_value":"紀要論文"}]},"item_3_text_44":{"attribute_name":"資源タイプ・NII","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper"}]},"item_3_text_45":{"attribute_name":"資源タイプ・DCMI","attribute_value_mlt":[{"subitem_text_value":"text"}]},"item_3_text_46":{"attribute_name":"資源タイプ・ローカル表示コード","attribute_value_mlt":[{"subitem_text_value":"2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"武田, 裕司"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"奈良, 英利"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"浅尾, 裕信"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-08-22"}],"displaytype":"detail","filename":"kiyoum-35-2-021to032.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kiyoum-35-2-021to032","url":"https://yamagata.repo.nii.ac.jp/record/4211/files/kiyoum-35-2-021to032.pdf"},"version_id":"8a15e4dc-a72a-45cb-ae26-351d6de9c9d4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Analysis of signal transducers using flow cytometry is useful for detection of contractive and fluctuating signals","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of signal transducers using flow cytometry is useful for detection of contractive and fluctuating signals"}]},"item_type_id":"3","owner":"17","path":["101"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-08-10"},"publish_date":"2017-08-10","publish_status":"0","recid":"4211","relation_version_is_last":true,"title":["Analysis of signal transducers using flow cytometry is useful for detection of contractive and fluctuating signals"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-05-25T06:32:24.221903+00:00"}