| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2010-06-25 |
| タイトル |
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|
タイトル |
Primary research on MGSAD023, a new homokogue of eIF4G |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
Pan, Lei
Yamamoto, Takashi
Kimura, Wataru
|
| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
Abstruct MGSAD023 (Mouse Gene Similar to AD023; Genbank accession AK150749) and its human compartment (AD023; Genbank accession AF225422) are new two genes with unknown functions. They were derived from high-throughput cDNA sequence analysis of the mouse genome project and Human Genome Project (HGP)，respec- tively. MGSAD023 shows 88.6% identity to AD023 (NCBI Blast)，and we decided to use MGSAD023 as a temple to explore the functions of these two compartment genes. With the NCBI Conserved Domain Database (CDD) and the gap program in GCG se- quence analysis software，we found that the amino acids of MGSAD023 showed 58.3% similarity and 4l.2% identity to eIF4G and also showed a good match to the middle domain of eIF4G. We determined the transcription pattern of MGSAD023. RT-PCR results showed that MGSAD023 was expressed in all of the tissues checked，including cerebellum， cerebra，liver，kidney，tongue，testis，heart and spleen，without any significant differ- ences.The results of In Situ Hybridization (ISH) showed that there was a very strong signal of mRNA of MGSAD023 on the seminiferous tubular basement membrane， where spermatogonia A and B are located. In silico exploration revealed homologous genes in various species，i ncluding chimpanzee，rat，mice，dog，zebra fish，fruit fly， xenopus，and cattle. These results hint that MGSAD023 is a conserved gene. It may act as a translation factor during cellular mitosis，especially in spermatogonia. |
| 書誌情報 |
山形大学紀要. 医学 : 山形医学 = Bulletin of the Yamagata University. Medical science : Yamagata medical journal
巻 27,
号 1,
p. 23-29,
発行日 2009-02-16
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| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00067279 |
| 出版者 |
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出版者 |
山形大学 |
| 出版者（ヨミ） |
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|
値 |
ヤマガタ ダイガク |
| 別言語の出版者 |
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|
値 |
Yamagata University |
| 資源タイプ |
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内容記述タイプ |
Other |
|
内容記述 |
論文(Article) |
| 資源タイプ・ローカル |
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|
値 |
紀要論文 |
| 資源タイプ・NII |
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値 |
Departmental Bulletin Paper |
| 資源タイプ・DCMI |
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|
値 |
text |
| 資源タイプ・ローカル表示コード |
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|
値 |
2 |
kiyoum-27-1-023to029.pdf (280.8 kB)
