Item type |
紀要論文 / Departmental Bulletin Paper(1) |
公開日 |
2010-06-25 |
タイトル |
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タイトル |
Primary research on MGSAD023, a new homokogue of eIF4G |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
著者 |
Pan, Lei
Yamamoto, Takashi
Kimura, Wataru
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内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
Abstruct MGSAD023 (Mouse Gene Similar to AD023; Genbank accession AK150749) and its human compartment (AD023; Genbank accession AF225422) are new two genes with unknown functions. They were derived from high-throughput cDNA sequence analysis of the mouse genome project and Human Genome Project (HGP),respec- tively. MGSAD023 shows 88.6% identity to AD023 (NCBI Blast),and we decided to use MGSAD023 as a temple to explore the functions of these two compartment genes. With the NCBI Conserved Domain Database (CDD) and the gap program in GCG se- quence analysis software,we found that the amino acids of MGSAD023 showed 58.3% similarity and 4l.2% identity to eIF4G and also showed a good match to the middle domain of eIF4G. We determined the transcription pattern of MGSAD023. RT-PCR results showed that MGSAD023 was expressed in all of the tissues checked,including cerebellum, cerebra,liver,kidney,tongue,testis,heart and spleen,without any significant differ- ences.The results of In Situ Hybridization (ISH) showed that there was a very strong signal of mRNA of MGSAD023 on the seminiferous tubular basement membrane, where spermatogonia A and B are located. In silico exploration revealed homologous genes in various species,i ncluding chimpanzee,rat,mice,dog,zebra fish,fruit fly, xenopus,and cattle. These results hint that MGSAD023 is a conserved gene. It may act as a translation factor during cellular mitosis,especially in spermatogonia. |
書誌情報 |
山形大学紀要. 医学 : 山形医学 = Bulletin of the Yamagata University. Medical science : Yamagata medical journal
巻 27,
号 1,
p. 23-29,
発行日 2009-02-16
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書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN00067279 |
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出版者 |
山形大学 |
出版者(ヨミ) |
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ヤマガタ ダイガク |
別言語の出版者 |
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Yamagata University |
資源タイプ |
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内容記述タイプ |
Other |
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内容記述 |
論文(Article) |
資源タイプ・ローカル |
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紀要論文 |
資源タイプ・NII |
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Departmental Bulletin Paper |
資源タイプ・DCMI |
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text |
資源タイプ・ローカル表示コード |
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2 |