Item type |
紀要論文 / Departmental Bulletin Paper(1) |
公開日 |
2011-10-06 |
タイトル |
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タイトル |
Investigation of liposome-conjugated Nuclear Factor-κB Decoy Oligodeoxynucleotide in a Mouse Model of Dextran Sodium Sulphate-induced Colitis |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
著者 |
鈴木, 康之
武田, 弘明
河田, 純男
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著者別名 |
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姓名 |
Suzuki, Yasukuni |
著者別名 |
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姓名 |
Takeda, Hiroaki |
著者別名 |
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姓名 |
Kawata, Sumio |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
BACKGROUNDS/AIMS: Double strand nuclear factor-κB decoy oligodeoxynucleotide (NF-κB-Decoy) had been investigated as a potential therapeutic approach in ulcerative colitis. Here we confirm that NF-κB-Decoy inhibits inflammatory cytokines production in vitro, and report initial studies of the use of liposome-conjugated NF-κB-Decoy to treat dextran sodium sulphate (DSS)-induced colitis. METHODS: (1) Peripheral blood samples or cultured Colo 205, colon cancer cells were pretreated with NF-κB-Decoy and stimulated with lipopolysaccharide. (2) Eight-weeks-old female BALB/C mice were fed 4% DSS in drinking water for 7 days. Intra-colonic administration of liposomeconjugated NF-κB-Decoy started at the same time as DSS treatment, and continued for 14 days. RESULTS: (1) In the peripheral blood, NF-κB-Decoy significantly inhibited interleukin (IL)-6 production (Tukey test, p<0.05). In the cultured cells, NF-κB-Decoy significantly reduced IL-8 production (t-test, p<0.01). (2) During administration of DSS, mortality rate between NF-κB-Decoy treated and control mice (72.4% and 57.6% respectively) was not significantly different, and body weight loss and histopathological dameges were similar in both groups. But, after discontinuation of DSS, NF-κB-Decoytreated mice regained body weight significantly earlier than did control mice. CONCLUSIONS: NF-κB-Decoy actually inhibited IL-6 and IL-8 production in vitro. Although liposome-conjugated NF-κB-Decoy could not show amelioration in DSS mice model, NF-κB-Decoy treated mice showed earlier regain of body weight. |
書誌情報 |
山形大学紀要. 医学 : 山形医学 = Bulletin of the Yamagata University. Medical science : Yamagata medical journal
巻 27,
号 2,
p. 109-117,
発行日 2009-08-15
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書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN00067279 |
その他の言語のタイトル |
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その他のタイトル |
デキストラン硫酸誘発マウス大腸炎に対するリポソーム封入NF-κBデコイオリゴデオキシリボ核酸の検討 |
タイトル(ヨミ) |
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その他のタイトル |
デキストラン リュウサン ユウハツ マウス ダイチョウエン ニ タイスル リポソーム フウニュウ NF-κB デコイ オリゴ デオキシリボ カクサン ノ ケントウ |
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出版者 |
山形大学 |
出版者(ヨミ) |
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ヤマガタ ダイガク |
別言語の出版者 |
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Yamagata University |
資源タイプ |
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内容記述タイプ |
Other |
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内容記述 |
論文(Article) |
資源タイプ・ローカル |
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紀要論文 |
資源タイプ・NII |
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Departmental Bulletin Paper |
資源タイプ・DCMI |
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text |
資源タイプ・ローカル表示コード |
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2 |